Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection

Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308ΔwbkA) and evaluated its virulence. The survival of 2308ΔwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308ΔwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308ΔwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.     

动物运输应激源与应激反应影响因子研究进展

动物的运输是畜牧业的重要生产环节,但运输活动带来的动物福利问题也越来越受到关注。开展动物运输应激源和应激反应影响因子的研究,对深入认识动物运输应激本质和提出解决方案乃至相关立法具有重要意义。论文对前人开展的动物运输应激源(拥挤、禁食禁水、气温、装卸、颠簸等)的分离研究和应激反应影响因子(运输距离和运输时间、年龄和经验、性情、恐惧信息素)的研究进行了回顾整理,并分析了争议的原因,为动物运输应激领域研究提供参考或指出新的研究方向。     

四川省部分地区山羊沙门氏菌健康带菌率及耐药性调查

为了解四川省山羊沙门氏菌带菌情况,本试验采集了四川省部分地区5个规模化山羊养殖场表观健康山羊粪便196份,经BPW预增菌、TTB选择性增菌后,采用靶向invA基因的PCR方法检测沙门氏菌的带菌率;对阳性样本进行细菌分离鉴定,随机选择25株沙门氏菌分离株测定其对15种抗菌药物的敏感性。结果显示,山羊沙门氏菌的平均带菌率为54.59%。药敏试验结果显示,分离株均对丁胺卡那敏感,而对其余14种抗菌药物呈不同程度的耐药,多重耐药菌株占88%,其中耐2~7种药物的占52%,耐9~13种药物的占36%。结果表明,四川省部分地区山羊的沙门氏菌健康带菌率较高,且多重耐药性普遍,其公共卫生意义值得进一步研究。     

Inductively coupled plasma mass‐spectrometric determination of platinum in excretion products of client‐owned pet dogs

Residues of antineoplastic drugs in canine excretion products may represent exposure risks to veterinary personnel, owners of pet dogs and other animal care‐takers. The aim of this study was to measure the extent and duration of platinum (Pt) excretion in pet dogs treated with carboplatin. Samples were collected before and up to 21 days after administration of carboplatin. We used validated, ultra‐sensitive, inductively coupled plasma‐mass spectrometry assays to measure Pt in canine urine, faeces, saliva, sebum and cerumen. Results showed that urine is the major route of elimination of Pt in dogs. In addition, excretion occurs via faeces and saliva, with the highest amounts eliminated during the first 5 days. The amount of excreted Pt decreased over time but was still quantifiable at 21 days after administration of carboplatin. In conclusion, increased Pt levels were found in all measured excretion products up to 21 days after administration of carboplatin to pet dogs, with urine as the main route of excretion. These findings may be used to further adapt current veterinary guidelines on safe handling of antineoplastic drugs and treated animals.     

Recurrent Temporary Paralysis Reported After Human Rabies Post‐Exposure Prophylaxis

Adverse events can occur after rabies post‐exposure prophylaxis (PEP), and linkage to causality is often difficult to determine. We report a case of recurrent temporary paralysis that began immediately after the initiation of rabies PEP in a man exposed to a bat. The recurrent temporary paralysis first occurred in the patient after his initial dose and then again after day 3 of his rabies PEP. The PEP was terminated prior to a serologic response. The patient continued to experience numerous discrete episodes of temporary paralysis for over two years.     

阔叶树落叶分解过程与土壤动物的作用

本文研究了东北３种阔叶树落叶的分解与土壤动物在其分解过程中的作用。结果表明，３种枯叶的重量损失速度排序：水曲柳＞白桦＞蒙古栎。３种枯叶的半分解期及分解９５％所需时间分别为：水曲柳０．４年与１．７年，白桦０．９年与３．９年，蒙古栎为１．９年与８．２年。在枯叶分解过程中起主要作用的大型土壤动物有蚯蚓、线蚓、马陆、腹足类、昆虫及其幼虫；中小型土壤动物有线虫、螨类、弹尾类等３０余种。     

云南野生动物养殖产业化初探

云南被誉为"动物王国",地理区位特殊,自然条件优越,物种资源丰富,开发潜力巨大,但野生动物养殖业却严重滞后,大资源、小产业、低效益的状况十分突出。针对产业发展中存在的问题,结合国内外市场需求,提出了产业开发的思路、方向及对策建议。     

马尾松毛虫安全区划分的动物多样性指数指标研究

通过比较广西宾阳县、宁明县、陆川县、临桂县、全州县马尾松毛虫安全区春季与夏季松林群落中的害虫种数、天敌种数、中性动物种数和益害比、动物多样性指数,认为划分马尾松毛虫安全区动物多样性指数的具体数值为≥2.300,据此进一步将安全区划分为基本稳定区、中等稳定区和高度稳定区.     

福建寿宁县兽害猖獗的成因及对策初探

1998～2006年,以寿宁县为调查对象,运用机械抽样法调查样带69条,运用访问调查法调查猎户农户100余户,运用点面互换法调查全县范围内农田作物受害程度。结果表明,全县近半数的村,15%的耕地存在兽害,99%的兽害及其损失源于野猪的活动。分析表明,兽害的成因有种群爆发、物种灭绝和生态系统衰败诸多因素,而人为干扰是各种现象的起因。最后提出治理山区兽害的对策,尤其应加强生态系统恢复和自然保护区建设。     

湖南借母溪自然保护区两栖动物区系研究

报道了借母溪自然保护区两栖动物资源和区系特征的调查结果。已知有2目8科25种。广布种5种，古北界种1种，东洋界19种。进而进行区系分析和多样性分析。